Recombinant human TK2 protein
TK2, also known as thymidine kinase 2 mitochondrial, belongs to the DCK-DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase) : 2-deoxythymidine kinase, ATP-thymidine 5-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2) . A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Thymidine kinase 2 mitochondrial, thymidine kinase 2, mitochondrial, MTDPS2, MTTK
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
30.1 kDa (257aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Saada A., et al. (2001) Nat. Genet. 29:342-344; ; Kit S. (1985) . Thymidine kinase. Microbiol. Sci. 2 (12) : 369-75.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl, 250 mM Imidazole, 0.1mM PMSF
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_004605.4
Uniprot Accession Number
O00142
Species
Human
AA Sequence
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