Recombinant human TK2 protein

TK2, also known as thymidine kinase 2 mitochondrial, belongs to the DCK-DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase) : 2-deoxythymidine kinase, ATP-thymidine 5-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2) . A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thymidine kinase 2 mitochondrial, thymidine kinase 2, mitochondrial, MTDPS2, MTTK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

30.1 kDa (257aa) confirmed by MALDI-TOF

Additionnal Information

TK2, thymidine kinase 2 mitochondrial, thymidine kinase 2, mitochondrial, MTDPS2, MTTK, ATGP1651-10 µg, ATGP1651-20 µg, ATGP1651-50 µg, ATGP1651-100 µg, ATGP1651-250 µg, ATGP1651-500 µg, ATGP1651-1 mg, ATGP1651-10, ATGP1651-20, ATGP1651-50, ATGP1651-100, ATGP1651-250, ATGP1651-500, ATGP1651-1

References & Citations

Saada A., et al. (2001) Nat. Genet. 29:342-344; ; Kit S. (1985) . Thymidine kinase. Microbiol. Sci. 2 (12) : 369-75.

Other References

Aoki H, et al. Thymidine Kinase 2 and Mitochondrial Protein COX I in the Cerebellum of Patients with Spinocerebellar Ataxia Type 31 Caused by Penta-nucleotide Repeats (TTCCA) n. (Cerebellum. 2022) {https://pubmed.ncbi.nlm.nih.gov/35084690/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl, 250 mM Imidazole, 0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004605.4

Uniprot Accession Number

O00142

Species

Human

AA Sequence

VQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP