Recombinant human JNK2/MAPK9 protein

Mitogen-activated protein kinase 9, also known as MAPK9, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK9, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors. Recombinant human MAPK9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, p54a, p54aSAPK, PRKM9, SAPK, SAPK1a

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

46.6 kDa (406aa) confirmed by MALDI-TOF

Additionnal Information

MAPK9, Mitogen-activated protein kinase 9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, p54a, p54aSAPK, PRKM9, SAPK, SAPK1a, ATGP1649-10 µg, ATGP1649-20 µg, ATGP1649-50 µg, ATGP1649-100 µg, ATGP1649-250 µg, ATGP1649-500 µg, ATGP1649-1 mg, ATGP1649-10, ATGP1649-20, ATGP1649-50, ATGP1649-100, ATGP1649-250, ATGP1649-500, ATGP1649-1

References & Citations

Kyriakis J M., et al. (1999) J Biol Chem. 274: 5259-62; ; Whitmarsh., et al. (1998) Trends Biochem Sci. 23: 481-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_620707.1

Uniprot Accession Number

P45984

Species

Human

AA Sequence

MSDSKC DSQFYSVQVA DSTFTVLKRY QQLKPIGSGA QGIVCAAFDT VLGINVAVKK LSRPFQNQTH AKRAYRELVL LKCVNHKNII SLLNVFTPQK TLEEFQDVYL VMELMDANLC QVIHMELDHE RMSYLLYQML CGIKHLHSAG IIHRDLKPSN IVVKSDCTLK ILDFGLARTA CTNFMMTPYV VTRYYRAPEV ILGMGYKENV DIWSVGCIMG ELVKGCVIFQ GTDHIDQWNK VIEQLGTPSA EFMKKLQPTV RNYVENRPKY PGIKFEELFP DWIFPSESER DKIKTSQARD LLSKMLVIDP DKRISVDEAL RHPYITVWYD PAEAEAPPPQ IYDAQLEERE HAIEEWKELI YKEVMDWEER SKNGVVKDQP SAQMQQ