Recombinant human CMBL protein
Carboxymethylenebutenolidase homolog, also known as CMBL, is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15. 2. Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Carboxymethylenebutenolidase homolog
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.6 kDa (269aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ravandi F., et al. (2009) Cancer. 115: 5746-5751; ; Ishizuka T., et al. (2010) J Biol Chem. 285: 11892-11902.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM EDTA
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_620164.1
Uniprot Accession Number
Q96DG6
Species
Human
AA Sequence
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