Recombinant human TMOD3 protein

TMOD3 belongs to the tropomodulin family. Regulation of the actin cytoskeleton by filament capping proteins is critical to myriad dynamic cellular functions. The ability of these proteins to bind both filaments as well as monomers is often central to their cellular functions. The ubiquitous pointed end capping protein TMOD3 acts as a negative regulator of cell migration, yet mechanisms behind its cellular functions are not understood. Recombinant human TMOD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tropomodulin-3, Tropomodulin 3, u-Tmod, uTMOD, ubiquitous tropomodulin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

42 kDa (376aa) confirmed by MALDI-TOF

Additionnal Information

TMOD3, Tropomodulin-3, Tropomodulin 3, u-Tmod, uTMOD, ubiquitous tropomodulin, ATGP1647-10 µg, ATGP1647-20 µg, ATGP1647-50 µg, ATGP1647-100 µg, ATGP1647-250 µg, ATGP1647-500 µg, ATGP1647-1 mg, ATGP1647-10, ATGP1647-20, ATGP1647-50, ATGP1647-100, ATGP1647-250, ATGP1647-500, ATGP1647-1

References & Citations

Fischer RS., et al. (2006) J Biol Chem. 2006 Nov 24; ;281 (47) :36454-65. Epub 2006 Oct 1.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_055362

Uniprot Accession Number

Q9NYL9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMALPFR KDLEKYKDLD EDELLGNLSE TELKQLETVL DDLDPENALL PAGFRQKNQT SKSTTGPFDR EHLLSYLEKE ALEHKDREDY VPYTGEKKGK IFIPKQKPVQ TFTEEKVSLD PELEEALTSA SDTELCDLAA ILGMHNLITN TKFCNIMGSS NGVDQEHFSN VVKGEKILPV FDEPPNPTNV EESLKRTKEN DAHLVEVNLN NIKNIPIPTL KDFAKALETN THVKCFSLAA TRSNDPVATA FAEMLKVNKT LKSLNVESNF ITGVGILALI DALRDNETLA ELKIDNQRQQ LGTAVELEMA KMLEENTNIL KFGYQFTQQG PRTRAANAIT KNNDLVRKRR VEGDHQ