Recombinant mouse PD-L1/B7-H1 protein

CD274, also known as programmed cell death 1 ligand 1 (PDCD1L1), is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. This protein interacts with PDCD1 inhibits T-cell proliferation and cytokine production. Recombinant mouse CD274 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

CD274 antigen, A530045L16Rik, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.6 kDa (246aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PD-L1, B7-H1, CD274 antigen, A530045L16Rik, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, ATGP1645-10 µg, ATGP1645-20 µg, ATGP1645-50 µg, ATGP1645-100 µg, ATGP1645-250 µg, ATGP1645-500 µg, ATGP1645-1 mg, ATGP1645-10, ATGP1645-20, ATGP1645-50, ATGP1645-100, ATGP1645-250, ATGP1645-500, ATGP1645-1

References & Citations

Li, X., et al. (2012) J. Immunol. 188 (2), 724-734; ; Pulko, V., et al. (2011) J. Immunol. 187 (11), 5606-5614

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_068693

Uniprot Accession Number

Q9EP73

Species

Mouse

AA Sequence

FTITA PKDLYVVEYG SNVTMECRFP VERELDLLAL VVYWEKEDEQ VIQFVAGEED LKPQHSNFRG RASLPKDQLL KGNAALQITD VKLQDAGVYC CIISYGGADY KRITLKVNAP YRKINQRISV DPATSEHELI CQAEGYPEAE VIWTNSDHQP VSGKRSVTTS RTEGMLLNVT SSLRVNATAN DVFYCTFWRS QPGQNHTAEL IIPELPATHP PQNRTH