Recombinant human ASF1B protein
ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 protein in yeast. This protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Recombinant human ASF1B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
23.4 kDa (210aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Corpet, A., et al. (2011) EMBO J. 30 (3), 480-493; ; Jasencakova, Z., et al. (2010) Mol. Cell 37 (5), 736-743
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_060624.1
Uniprot Accession Number
Q9NVP2
Species
Human
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