Recombinant human TTC1 protein
TTC1, also known as tetratricopeptide repeat protein 1, is composed of 292 amino acids with three TPR motifs, as a Galpha16-binding protein. The interaction was confirmed both in vitro and in transfected mammalian cells, where TTC1 also binds to several other Galpha proteins. TTC1 was found to interact with Ha-Ras preferentially in its active form. Recombinant human TTC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Tetratricopeptide repeat protein 1, TPR1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
36.1 kDa (316aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Liu AM., et al. (2011) BMC Struct Biol. 13; ;11:17; ; Strausberg RL., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 26:16899-16903
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_003305
Uniprot Accession Number
Q99614
Species
Human
AA Sequence
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