Recombinant human SERTAD1 protein
SERTAD1, also known as SERTA domain-containing protein, acts at E2F-responsive promoters to integrate signals provided by PHD- and/or bromodomain-containing transcription factors. This protein stimulates E2F-1/DP-1 transcriptional activity. Recombinant human SERTAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
SERTA domain-containing protein, SEI1, TRIP-Br1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
27.3 kDa (260aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Hsu S.-I., et al. (2001) EMBO J. 20:2273-2285; ; Sugimoto M., et al. (1999) Genes Dev. 13:3027-3033
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_037508.2
Uniprot Accession Number
Q9UHV2
Species
Human
AA Sequence
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