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Recombinant human DNAJC12 protein

DNAJC12, also known as JDP1, is a member of the DnaJ/Hsp40 family. The members of the DnaJ/Hsp40 proteins are highly conserved through evolution, expressed in several tissues and act as co-chaperone regulating protein folding, transport, translational initiation and gene expression. DnaJC12 may play a role in protein folding and export and is thought to be involved in estrogen control and the development of breast cancer. This protein is expressed at high levels in brain, heart, and testis, and at reduced levels in kidney and stomach. Recombinant human DNAJC12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DnaJ (Hsp40) homolog subfamily C member 12, DnaJ (Hsp40) homolog, subfamily C, member 12, JDP1, RP11-57G10.2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26kDa (222aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

DNAJC12, DnaJ (Hsp40) homolog subfamily C member 12, DnaJ (Hsp40) homolog, subfamily C, member 12, JDP1, RP11-57G10.2, ATGP1636-10 µg, ATGP1636-20 µg, ATGP1636-50 µg, ATGP1636-100 µg, ATGP1636-250 µg, ATGP1636-500 µg, ATGP1636-1 mg, ATGP1636-10, ATGP1636-20, ATGP1636-50, ATGP1636-100, ATGP1636-250, ATGP1636-500, ATGP1636-1

References & Citations

De Bessa SA, et al. (2006) J Mol Med. 17 (2) :363-7.; ; Mahlknecht, u., et al. (2009) Int. J. Mol. Med. 23: 245-252.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_068572

Uniprot Accession Number

Q9UKB3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMDAILN YRSEDTEDYY TLLGCDELSS VEQILAEFKV RALECHPDKH PENPKAVETF QKLQKAKEIL TNEESRARYD HWRRSQMSMP FQQWEALNDS VKTSMHWVVR GKKDLMLEES DKTHTTKMEN EECNEQRERK KEELASTAEK TEQKEPKPLE KSVSPQNSDS SGFADVNGWH LRFRWSKDAP SELLRKFRNY EI

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