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Recombinant human ADPRH protein

ADPRH (ADP-ribosylarginine hydrolase), also known as ADP-ribose-L-arginine cleaving enzyme, belongs to the ADP-ribosylglycohydrolase family. This protein catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent. Recombinant human ADPRH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein ADP-ribosylarginine hydrolase, ARH1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

42.1 kDa (381aa) confirmed by MALDI-TOF

Additionnal Information

ADPRH, Protein ADP-ribosylarginine hydrolase, ARH1, ATGP1633-10 µg, ATGP1633-20 µg, ATGP1633-50 µg, ATGP1633-100 µg, ATGP1633-250 µg, ATGP1633-500 µg, ATGP1633-1 mg, ATGP1633-10, ATGP1633-20, ATGP1633-50, ATGP1633-100, ATGP1633-250, ATGP1633-500, ATGP1633-1

References & Citations

Weber F, et al. (2005) J Clin Endocrinol Metab. 90 (2) :1149-55.; ; Kernstock S, et al. (2009) Acta Crystallogr Sect F Struct Biol Cryst Commun. 65 (Pt 5) :529-32.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001116

Uniprot Accession Number

P54922

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMEKYVA AMVLSAAGDA LGYYNGKWEF LQDGEKIHRQ LAQLGGLDAL DVGRWRVSDD TVMHLATAEA LVEAGKAPKL TQLYYLLAKH YQDCMEDMDG RAPGGASVHN AMQLKPGKPN GWRIPFNSHE GGCGAAMRAM CIGLRFPHHS QLDTLIQVSI ESGRMTHHHP TGYLGALASA LFTAYAVNSR PPLQWGKGLM ELLPEAKKYI VQSGYFVEEN LQHWSYFQTK WENYLKLRGI LDGESAPTFP ESFGVKERDQ FYTSLSYSGW GGSSGHDAPM IAYDAVLAAG DSWKELAHRA FFHGGDSDST AAIAGCWWGV MYGFKGVSPS NYEKLEYRNR LEETARALYS LGSKEDTVIS L

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