Recombinant human Crystallin gamma N/CRYGN protein

Crystallin, gamma N, also known as CRYGN, belongs to the Crystallins family. Crystallins are divided into alpha, beta and gamma families, and the beta and gamma-crystallins also comprise a superfamily. Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. The CRYGN is differentially regulated after early development, and are involved in cataract formation as a result of either age-related protein degradation or genetic mutation. Recombinant human CRYGN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Gamma-crystallin N, Gamma-N-crystallin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.1 kDa (206aa)

Additionnal Information

CRYGN, Crystallin gamma N, Crystallin, gamma N, gamma-crystallin N, ATGP1627-10 µg, ATGP1627-20 µg, ATGP1627-50 µg, ATGP1627-100 µg, ATGP1627-250 µg, ATGP1627-500 µg, ATGP1627-1 mg, ATGP1627-10, ATGP1627-20, ATGP1627-50, ATGP1627-100, ATGP1627-250, ATGP1627-500, ATGP1627-1

References & Citations

Srivastava O P., et al. (1998) Curr Eye Res. 17:1074-1081.; ; Srivastava O P., et al. (1998) Biophys Res Commun. 253:288-294.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_653328

Uniprot Accession Number

Q8WXF5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAQRSG KITLYEGKHF TGQKLEVFGD CDNFQDRGFM NRVNSIHVES GAWVCFNHPD FRGQQFILEH GDYPDFFRWN SHSDHMGSCR PVGMHGEHFR LEIFEGCNFT GQCLEFLEDS PFLQSRGWVK NCVNTIKVYG DGAAWSPRSF GAEDFQLSSS LQSDQGPEEA TTKPATTQPP FLTANL