Recombinant human Catalase protein

CAT is a key antioxidant enzyme in the bodies defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 100,000 units/mg. One unit will decompose 1.0umole of H2O2 per minute at pH 8.0 at 25C.

Product Name Alternative

CAT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 100,000unit/mg. One unit will decompose 1.0umole of H2O2 per minute at pH 8.0 at 25C.

Molecular Weight

61.9 kDa (547aa)

Additionnal Information

Catalase, ATGP1624-10 µg, ATGP1624-20 µg, ATGP1624-50 µg, ATGP1624-100 µg, ATGP1624-250 µg, ATGP1624-500 µg, ATGP1624-1 mg, ATGP1624-10, ATGP1624-20, ATGP1624-50, ATGP1624-100, ATGP1624-250, ATGP1624-500, ATGP1624-1

References & Citations

Freitas MO, et al. (2011) J Biol Chem. 2011 Nov 25; ;286 (47) :40509-19. Epub 2011 Oct 5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_001743

Uniprot Accession Number

P04040

Species

Human

AA Sequence

MADSRDPASD QMQHWKEQRA AQKADVLTTG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF EHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNANGEAV YCKFHYKTDQ GIKNLSVEDA ARLSQEDPDY GIRDLFNAIA TGKYPSWTFY IQVMTFNQAE TFPFNPFDLT KVWPHKDYPL IPVGKLVLNR NPVNYFAEVE QIAFDPSNMP PGIEASPDKM LQGRLFAYPD THRHRLGPNY LHIPVNCPYR ARVANYQRDG PMCMQDNQGG APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRLC ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA AREKANL