Recombinant human Catalase protein
CAT is a key antioxidant enzyme in the bodies defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 100,000 units/mg. One unit will decompose 1.0umole of H2O2 per minute at pH 8.0 at 25C.
Product Name Alternative
CAT
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 100,000unit/mg. One unit will decompose 1.0umole of H2O2 per minute at pH 8.0 at 25C.
Molecular Weight
61.9 kDa (547aa)
Additionnal Information
References & Citations
Freitas MO, et al. (2011) J Biol Chem. 2011 Nov 25; ;286 (47) :40509-19. Epub 2011 Oct 5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_001743
Uniprot Accession Number
P04040
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
Rims4 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
ELISA Kit For Phosphoribosyl pyrophosphate synthase-associated protein 2
