Recombinant human CD16a/FCGR3A protein

Low affinity immunoglobulin gamma Fc region receptor III-A (FCGR3A), also known as CD16A. This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other antibody-dependent responses. FCGR3A requires association of the gamma subunit of Fc epsilon RI or the zeta subunit of the TCR-CD3 complex for cell surface expression. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Recombinant human FCGR3A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Low affinity immunoglobulin gamma Fc region receptor III-A, low affinity immunoglobulin gamma Fc region receptor III-A isoform c, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, FCGRIII, IGFR3, IMD20, CD16

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26 kDa (228aa)

Additionnal Information

Fc g RIIIA, CD16a, Low affinity immunoglobulin gamma Fc region receptor III-A, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, ATGP1623-10 µg, ATGP1623-20 µg, ATGP1623-50 µg, ATGP1623-100 µg, ATGP1623-250 µg, ATGP1623-500 µg, ATGP1623-1 mg, ATGP1623-10, ATGP1623-20, ATGP1623-50, ATGP1623-100, ATGP1623-250, ATGP1623-500, ATGP1623-1

References & Citations

Huizinga T W., et al. (1988) Nature. 333:667-669.; ; Nagarajan S., et al. (1995) J Biol Chem. 270:25762-25770.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001121065

Uniprot Accession Number

P08637

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRT EDLPKAVVFL EPQWYRVLEK DSVTLKCQGA YSPEDNSTQW FHNESLISSQ ASSYFIDAAT VDDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKGRKY FHHNSDFYIP KATLKDSGSY FCRGLFGSKN VSSETVNITI TQGLAVSTIS SFFPPGYQ