Recombinant human DIMT1 protein

DIMT1 (DIM1 dimethyladenosine transferase 1 homolog) belongs to the methyltransferase superfamily. This enzyme is specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. It functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. DIMT1L is localized to the nucleolus. Recombinant human DIMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DIM1 dimethyladenosine transferase 1 homolog, DIM1, DIMT1L, HSA9761, HuSSY5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.5 kDa (334aa) confirmed by MALDI-TOF

Additionnal Information

DIMT1, DIM1 dimethyladenosine transferase 1 homolog, DIM1, DIMT1L, HSA9761, HuSSY5, ATGP1622-10 µg, ATGP1622-20 µg, ATGP1622-50 µg, ATGP1622-100 µg, ATGP1622-250 µg, ATGP1622-500 µg, ATGP1622-1 mg, ATGP1622-10, ATGP1622-20, ATGP1622-50, ATGP1622-100, ATGP1622-250, ATGP1622-500, ATGP1622-1

References & Citations

Scherl A., et al. (2002) Mol. Biol. Cell 13:4100-4109; ; Saltman, et al. (1993) Genomics 16:726-732.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 2mM DTT, 200 mM NaCl, 2mM EDTA

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_055288

Uniprot Accession Number

Q9UNQ2

Species

Human

AA Sequence

MPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS