Recombinant human EMG1 protein

EMG1 nucleolar protein homolog, also known as EMG1, is a 244 amino acid protein that is thought to be involved in ribosome biogenesis. Localized to the nucleolus, EMG1 participates in pre-18S rRNA processing and may play an important role in the assembly of the small ribosomal subunit, possibly controlling methylation during ribosome synthesis. A mutation in this gene has been associated with Bowen-Conradi syndrome. Recombinant human EMG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribosomal RNA small subunit methyltransferase NEP1, C2F, Grcc2f, NEP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.1 kDa (267aa) confirmed by MALDI-TOF

Additionnal Information

EMG1, Ribosomal RNA small subunit methyltransferase NEP1, C2F, Grcc2f, NEP1, ATGP1617-10 µg, ATGP1617-20 µg, ATGP1617-50 µg, ATGP1617-100 µg, ATGP1617-250 µg, ATGP1617-500 µg, ATGP1617-1 mg, ATGP1617-10, ATGP1617-20, ATGP1617-50, ATGP1617-100, ATGP1617-250, ATGP1617-500, ATGP1617-1

References & Citations

Buchhaupt M., et al. (2001) Mol Genet Genomics. 276: 273-284; ; Liu P C., et al. (2001) Mol Biol Cell. 12: 3644-3657.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006322

Uniprot Accession Number

Q92979

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI