Recombinant human DHODH protein
DHODH, also known as dihydroorotate dehydrogenase (quinone), mitochondrial, belongs to the dihydroorotate dehydrogenase family. DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Recombinant human DHODH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Dihydroorotate dehydrogenase (quinone) mitochondrial, Dihydroorotate dehydrogenase (quinone), mitochondrial, DHOdehase, POADS, uRA1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
42.3 kDa (390aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Rawls J., et al. (2000) Eur. J. Biochem. 267:2079-2087; ; Liu S., et al. (2000) Structure. 8:25-33
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001352
Uniprot Accession Number
Q02127
Species
Human
AA Sequence
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