Recombinant human DHODH protein

DHODH, also known as dihydroorotate dehydrogenase (quinone), mitochondrial, belongs to the dihydroorotate dehydrogenase family. DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. Recombinant human DHODH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Dihydroorotate dehydrogenase (quinone) mitochondrial, Dihydroorotate dehydrogenase (quinone), mitochondrial, DHOdehase, POADS, uRA1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

42.3 kDa (390aa) confirmed by MALDI-TOF

Additionnal Information

DHODH, Dihydroorotate dehydrogenase (quinone) mitochondrial, Dihydroorotate dehydrogenase (quinone), mitochondrial, DHOdehase, POADS, uRA1, ATGP1615-10 µg, ATGP1615-20 µg, ATGP1615-50 µg, ATGP1615-100 µg, ATGP1615-250 µg, ATGP1615-500 µg, ATGP1615-1 mg, ATGP1615-10, ATGP1615-20, ATGP1615-50, ATGP1615-100, ATGP1615-250, ATGP1615-500, ATGP1615-1

References & Citations

Rawls J., et al. (2000) Eur. J. Biochem. 267:2079-2087; ; Liu S., et al. (2000) Structure. 8:25-33

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_001352

Uniprot Accession Number

Q02127

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTGDER FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG IDGLIVTNTT VSRPAGLQGA LRSETGGLSG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV VGKVKRELEA LLKEQGFGGV TDAIGADHRR