Recombinant human APIP protein

APIP, also known as probable methylthioribulose-1-phosphate dehydratase, catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2, 3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) . It has an anti-apoptotic function and prevents muscle ischemic damage. This protein inhibits the cytochrome c-dependent and APAF1-mediated cell death. Recombinant human APIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Probable methylthioribulose-1-phosphate dehydratase, APIP2, CGI-29, CGI29, dJ179L10.2, MMRP19

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

29.7 kDa (266aa) confirmed by MALDI-TOF

Additionnal Information

APIP, Probable methylthioribulose-1-phosphate dehydratase, APIP2, CGI-29, CGI29, dJ179L10.2, MMRP19, ATGP1614-10 µg, ATGP1614-20 µg, ATGP1614-50 µg, ATGP1614-100 µg, ATGP1614-250 µg, ATGP1614-500 µg, ATGP1614-1 mg, ATGP1614-10, ATGP1614-20, ATGP1614-50, ATGP1614-100, ATGP1614-250, ATGP1614-500, ATGP1614-1

References & Citations

Cho D.-H., et al. (2004) J. Biol. Chem. 279:39942-39950; ; Lai C.-H., et al. (2000) Genome Res. 10:703-713

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_057041

Uniprot Accession Number

Q96GX9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV