Recombinant mouse S100A15 protein
Mouse S100A15 is a protein of 104 amino acids with a predicted molecular weight of 12, 870 Da and two EF-hand calcium binding sites. It is expressed in both skin and keratinocytes. S100A15 expression is upregulated in cultured keratinocytes induced to differentiate by calcium or phorbol esters. Recombinant mouse S100A15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
S100 calcium binding protein A15, AY465109, Gm1020, S100a7a, S100a15a, S100a17l1, S100A7f, S100A7L1
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15 kDa (132aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wolf R., et al. (2006) . J. Invest. Dermatol. 126 (7) : 1600-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_955454
Uniprot Accession Number
Q6S5I3
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMPDTPV EDSLFQIIHC FHHYAAREGD KETLSLEELK ALLLDSVPRF MDTLGRRQPY YITELFRAAD KNKDNQICFD EFLYILGKLV KDYHLQFHRQ LCAHYCTEHS LY
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