Recombinant human Ubc6/UBE2J2 protein

UBE2J2, also known as ubiquitin-conjugating enzyme E2 J2, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Recombinant human uBE2J2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Uubiquitin-conjugating enzyme E2 J2 isoform 2, Ubiquitin-conjugating enzyme E2, J2, UBC6 homolog, yeast, Ubc6p, UBC6, NCUBE2, E2 ubiquitin-conjugating enzyme J2, Non-canonical ubiquitin-conjugating enzyme 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28kDa (250aa) confirmed by MALDI-TOF

Additionnal Information

UBE2J2, ubiquitin-conjugating enzyme E2 J2, NCuBE-2, NCuBE2, PRO2121, ATGP1608-10 µg, ATGP1608-20 µg, ATGP1608-50 µg, ATGP1608-100 µg, ATGP1608-250 µg, ATGP1608-500 µg, ATGP1608-1 mg, ATGP1608-10, ATGP1608-20, ATGP1608-50, ATGP1608-100, ATGP1608-250, ATGP1608-500, ATGP1608-1

References & Citations

Wang X, et al. (2009) J Cell Biol. 187 (5) :655-68.; ; Nandi, D. et al. (2006) Journal of biosciences 31 (1) : 137-55.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 1mM DTT, 200 mM NaCl

Scientific Category

Ubiquitination

NCBI Accession Number

NP_477515

Uniprot Accession Number

Q8N2K1

Species

Human

AA Sequence

MSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH