Recombinant human HSCB protein

HSCB belongs to the hscB family and contains 1 J domain. This protein is expressed in lung, brain, stomach, spleen, ovary, testis, liver, muscle and heart and localized to mitochondria and cytoplasm. It may act as a co-chaperone in iron-sulfur cluster assembly in mitochondria, Interacts with ISCu and HSPA9. Recombinant human HSCB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

HscB iron-sulfur cluster co-chaperone homolog (E.coli), dJ366L4.2, DNAJC20, HSC20, JAC1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.7 kDa (231aa) confirmed by MALDI-TOF

Additionnal Information

HSCB, HscB iron-sulfur cluster co-chaperone homolog (E.coli), dJ366L4.2, DNAJC20, HSC20, JAC1, ATGP1604-10 µg, ATGP1604-20 µg, ATGP1604-50 µg, ATGP1604-100 µg, ATGP1604-250 µg, ATGP1604-500 µg, ATGP1604-1 mg, ATGP1604-10, ATGP1604-20, ATGP1604-50, ATGP1604-100, ATGP1604-250, ATGP1604-500, ATGP1604-1

References & Citations

Sun G., et al. (2003) J. Hum. Genet. 48, 415-419; ; Bitto E., et al. (2008) J. Biol. Chem. 283, 30184-30192

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_741999

Uniprot Accession Number

Q8IWL3

Species

Human

AA Sequence

AASQA GSNYPRCWNC GGPWGPGRED RFFCPQCRAL QAPDPTRDYF SLMDCNRSFR VDTAKLQHRY QQLQRLVHPD FFSQRSQTEK DFSEKHSTLV NDAYKTLLAP LSRGLYLLKL HGIEIPERTD YEMDRQFLIE IMEINEKLAE AESEAAMKEI ESIVKAKQKE FTDNVSSAFE QDDFEEAKEI LTKMRYFSNI EEKIKLKKIP L