Recombinant human PDCL protein

PDCL, also known as phosducin-like protein, belongs to the phosducin family. Phosducin-like protein is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin, a phosphoprotein expressed in retina and pineal gland. Both phosducin-like protein and phosphoducin have been shown to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. Recombinant human PDCL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Phosducin-like protein, PhLP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.8 kDa (325aa) confirmed by MALDI-TOF

Additionnal Information

PDCL, Phosducin-like protein, PhLP, ATGP1602-10 µg, ATGP1602-20 µg, ATGP1602-50 µg, ATGP1602-100 µg, ATGP1602-250 µg, ATGP1602-500 µg, ATGP1602-1 mg, ATGP1602-10, ATGP1602-20, ATGP1602-50, ATGP1602-100, ATGP1602-250, ATGP1602-500, ATGP1602-1

References & Citations

Thibault C., et al. (1999) Biochim. Biophys. Acta. 1444:346-354; ; Olsen J.V., et al. (2006) Cell. 127:635-648

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005379

Uniprot Accession Number

Q13371

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTTLDD KLLGEKLQYY YSSSEDEDSD HEDKDRGRCA PASSSVPAEA ELAGEGISVN TGPKGVINDW RRFKQLETEQ REEQCREMER LIKKLSMTCR SHLDEEEEQQ KQKDLQEKIS GKMTLKEFAI MNEDQDDEEF LQQYRKQRME EMRQQLHKGP QFKQVFEISS GEGFLDMIDK EQKSIVIMVH IYEDGIPGTE AMNGCMICLA AEYPAVKFCK VKSSVIGASS QFTRNALPAL LIYKGGELIG NFVRVTDQLG DDFFAVDLEA FLQEFGLLPE KEVLVLTSVR NSATCHSEDS DLEID