Recombinant human XAB1 protein

XAB1, as known as GPN1, belongs to the GPN-loop GTPase family. Small GTPases share a biochemical mechanism and act as binary molecular switches. One important function of small GTPases in the cell is nucleocytoplasmic transport of both proteins and RNA. This protein may play a role in DNA repair and may function in activation of transcription. And it forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Recombinant human XAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

XPA binding protein 1 GTPase, XPA binding protein 1, GTPase, GPN1, ATPBD1A, MBDIN, NTPBP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.3 kDa (398aa) confirmed by MALDI-TOF

Additionnal Information

XAB1, XPA binding protein 1 GTPase, XPA binding protein 1, GTPase, GPN1, ATPBD1A, MBDIN, NTPBP, ATGP1601-10 µg, ATGP1601-20 µg, ATGP1601-50 µg, ATGP1601-100 µg, ATGP1601-250 µg, ATGP1601-500 µg, ATGP1601-1 mg, ATGP1601-10, ATGP1601-20, ATGP1601-50, ATGP1601-100, ATGP1601-250, ATGP1601-500, ATGP1601-1

References & Citations

Carre C, et al. (2011) Mol Cell Biol. 31 (19) :3953-62.; ; Forget D, et al. (2010) Mol Cell Proteomics. 9 (12) :2827-39.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_009197

Uniprot Accession Number

Q9HCN4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAASAA AAELQASGGP RHPVCLLVLG MAGSGKTTFV QRLTGHLHAQ GTPPYVINLD PAVHEVPFPA NIDIRDTVKY KEVMKQYGLG PNGGIVTSLN LFATRFDQVM KFIEKAQNMS KYVLIDTPGQ IEVFTWSASG TIITEALASS FPTVVIYVMD TSRSTNPVTF MSNMLYACSI LYKTKLPFIV VMNKTDIIDH SFAVEWMQDF EAFQDALNQE TTYVSNLTRS MSLVLDEFYS SLRVVGVSAV LGTGLDELFV QVTSAAEEYE REYRPEYERL KKSLANAESQ QQREQLERLR KDMGSVALDA GTAKDSLSPV LHPSDLILTR GTLDEEDEEA DSDTDDIDHR VTEESHEEPA FQNFMQESMA QYWKRNNK