Recombinant human RYBP protein

RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG) . This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RING1 and YY1 binding protein, AAP1, DEDAF, YEAF1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

27.4 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

RYBP, RING1 and YY1 binding protein, AAP1, DEDAF, YEAF1, ATGP1600-10 µg, ATGP1600-20 µg, ATGP1600-50 µg, ATGP1600-100 µg, ATGP1600-250 µg, ATGP1600-500 µg, ATGP1600-1 mg, ATGP1600-10, ATGP1600-20, ATGP1600-50, ATGP1600-100, ATGP1600-250, ATGP1600-500, ATGP1600-1

References & Citations

Chen D, et al. (2008) EMBO Rep. 10 (2) :166-72. ; ; Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100 mM NaCl

Scientific Category

Apoptosis

NCBI Accession Number

NP_036366

Uniprot Accession Number

Q8N488

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF