Recombinant human RYBP protein
RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG) . This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
RING1 and YY1 binding protein, AAP1, DEDAF, YEAF1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
27.4 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Chen D, et al. (2008) EMBO Rep. 10 (2) :166-72. ; ; Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100 mM NaCl
Scientific Category
Apoptosis
NCBI Accession Number
NP_036366
Uniprot Accession Number
Q8N488
Species
Human
AA Sequence
Frequently Asked Questions
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