Recombinant human CKMT2 protein
CKMT2, as known as SMTCK, belongs to the ATP:guanido phosphotransferase family. SMTCK is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. This enzyme is reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e. g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Recombinant human CKMT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Creatine kinase S-type mitochondrial, Creatine kinase S-type, mitochondrial, SMTCK
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
46.1 kDa (405aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Payne RM, et al. (1995) Mol. Cell. Biochem. 133-134: 235-43. ; ; Qin W, Khuchua Z, et al. (1998) Mol. Cell. Biochem. 184 (1-2) : 153-67.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_001093205
Uniprot Accession Number
P17540
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
