Recombinant human DSTN protein
Destrin (actin depolymerizing factor), also known as DSTN, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. DSTN is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. DSTN shares 71% sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. Recombinant human DSTN protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Destrin (actin depolymerizing factor), ACTDP, ADF, bA462D18.2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
19.5 kDa (173aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nishida E., et al. (1985) Biochemistry. 24:6624-6630.; ; Kanamori T., et al. (1995) J Biol Chem. 270: 8061-8067.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Scientific Category
Actin & Myosin Related
NCBI Accession Number
NP_006861
Uniprot Accession Number
P60981
Species
Human
AA Sequence
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