Recombinant human VPS22/SNF8 protein

SNF 8, also known as EAP30 (ELL-associated protein of 30 kDa), is localizes to both the nucleus and the cytoplasm and is a member of the SNF8 family of vacuolar sorting proteins. This protein is subunit of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Recombinant human SNF8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SNF8 subunit of ESCRT-II, Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, EAP30, ESCRT-II complex subunit VPS22, hVps22, VPS22, Dot3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.4 kDa (282aa) confirmed by MALDI-TOF

Additionnal Information

SNF8, Vacuolar-sorting protein SNF8, Dot3, EAP30, VPS22, ATGP1594-10 µg, ATGP1594-20 µg, ATGP1594-50 µg, ATGP1594-100 µg, ATGP1594-250 µg, ATGP1594-500 µg, ATGP1594-1 mg, ATGP1594-10, ATGP1594-20, ATGP1594-50, ATGP1594-100, ATGP1594-250, ATGP1594-500, ATGP1594-1

References & Citations

Maleroed L., et al. (2007) Traffic 8:1617-1629; ; Raiborg C., et al. (2008) Exp. Cell Res. 314:801-813

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 2mM DTT, 200 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_009172.2

Uniprot Accession Number

Q96H20

Species

Human

AA Sequence

HRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP