Recombinant human VRK3 protein

VRK3, also known as inactive serine/threonine-protein kinase, belongs to the protein kinase superfamily. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. VRK3 is widely expressed in human tissues and its protein localizes to the nucleus. Recombinant human VRK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Inactive serine/threonine-protein kinase VRK3, Vaccinia related kinase 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

55.3 kDa (497aa)

Additionnal Information

VRK3, Inactive serine/threonine-protein kinase VRK3, Vaccinia related kinase 3, ATGP1585-10 µg, ATGP1585-20 µg, ATGP1585-50 µg, ATGP1585-100 µg, ATGP1585-250 µg, ATGP1585-500 µg, ATGP1585-1 mg, ATGP1585-10, ATGP1585-20, ATGP1585-50, ATGP1585-100, ATGP1585-250, ATGP1585-500, ATGP1585-1

References & Citations

Nichols R.J., et al. (2004) J. Biol. Chem. 279:7934-7946; ; Scheeff E.D., et al. (2009) Structure. 17:128-138

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057524

Uniprot Accession Number

Q8IV63

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMISFCPD CGKSIQAAFK FCPYCGNSLP VEEHVGSQTF VNPHVSSFQG SKRGLNSSFE TSPKKVKWSS TVTSPRLSLF SDGDSSESED TLSSSERSKG SGSRPPTPKS SPQKTRKSPQ VTRGSPQKTS CSPQKTRQSP QTLKRSRVTT SLEALPTGTV LTDKSGRQWK LKSFQTRDNQ GILYEAAPTS TLTCDSGPQK QKFSLKLDAK DGRLFNEQNF FQRAAKPLQV NKWKKLYSTP LLAIPTCMGF GVHQDKYRFL VLPSLGRSLQ SALDVSPKHV LSERSVLQVA CRLLDALEFL HENEYVHGNV TAENIFVDPE DQSQVTLAGY GFAFRYCPSG KHVAYVEGSR SPHEGDLEFI SMDLHKGCGP SRRSDLQSLG YCMLKWLYGF LPWTNCLPNT EDIMKQKQKF VDKPGPFVGP CGHWIRPSET LQKYLKVVMA LTYEEKPPYA MLRNNLEALL QDLRVSPYDP IGLPMVP