Recombinant human Calponin 1 protein

CNN1, also known as calponin-1, belongs to the calponin family. CNN1 is thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Recombinant human CNN1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Calponin 1, CNN1, HEL-S-14, Sm-Calp, SMCC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.2 kDa (305aa) confirmed by MALDI-TOF

Additionnal Information

SMCC, Sm-Calp, Sm Calp, HEL-S-14, HEL S 14, CNN-1, CNN1, CNN 1, Calponin-1, Calponin1, Calponin 1, ATGP1584-10 µg, ATGP1584-20 µg, ATGP1584-50 µg, ATGP1584-100 µg, ATGP1584-250 µg, ATGP1584-500 µg, ATGP1584-1 mg, ATGP1584-010, ATGP1584-020, ATGP1584-050, ATGP1584-100, ATGP1584-250, ATGP1584-500, ATGP1584-01M

References & Citations

Maguchi M., et al. (1995) Biochem. Biophys. Res. Commun. 217:238-244; ; Miano J.M., et al. (1997) Gene. 197:215-224

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Actin & Myosin Related

NCBI Accession Number

NP_001290

Uniprot Accession Number

P51911

Species

Human

AA Sequence

MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSALEH HHHHH