Recombinant human Cyclin B2 protein

CCNB2, also known as G2/mitotic-specific cyclin-B2, belongs to the cyclin family. CCNB2 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF) . Recombinant human CCNB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

G2/mitotic-specific cyclin-B2, HsT17299

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.8 kDa (422aa) confirmed by MALDI-TOF

Additionnal Information

Cyclin B2, G2/mitotic-specific cyclin-B2, HsT17299, ATGP1583-10 µg, ATGP1583-20 µg, ATGP1583-50 µg, ATGP1583-100 µg, ATGP1583-250 µg, ATGP1583-500 µg, ATGP1583-1 mg, ATGP1583-10, ATGP1583-20, ATGP1583-50, ATGP1583-100, ATGP1583-250, ATGP1583-500, ATGP1583-1

References & Citations

Wang B., et al. (2008) Anal. Chem. 80:9526-9533; ; Dephoure N., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:10762-10767

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 0.5M NaCl, 250 mM Imidazol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004692.1

Uniprot Accession Number

O95067

Species

Human

AA Sequence

HMALLRR PTVSSDLENI DTGVNSKVKS HVTIRRTVLE EIGNRVTTRA AQVAKKAQNT KVPVQPTKTT NVNKQLKPTA SVKPVQMEKL APKGPSPTPE DVSMKEENLC QAFSDALLCK IEDIDNEDWE NPQLCSDYVK DIYQYLRQLE VLQSINPHFL DGRDINGRMR AILVDWLVQV HSKFRLLQET LYMCVGIMDR FLQVQPVSRK KLQLVGITAL LLASKYEEMF SPNIEDFVYI TDNAYTSSQI REMETLILKE LKFELGRPLP LHFLRRASKA GEVDVEQHTL AKYLMELTLI DYDMVHYHPS KVAAAASCLS QKVLGQGKWN LKQQYYTGYT ENEVLEVMQH MAKNVVKVNE NLTKFIAIKN KYASSKLLKI SMIPQLNSKA VKDLASPLIG RS