Recombinant human Cyclin B2 protein
CCNB2, also known as G2/mitotic-specific cyclin-B2, belongs to the cyclin family. CCNB2 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF) . Recombinant human CCNB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
G2/mitotic-specific cyclin-B2, HsT17299
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.8 kDa (422aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wang B., et al. (2008) Anal. Chem. 80:9526-9533; ; Dephoure N., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:10762-10767
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 0.5M NaCl, 250 mM Imidazol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004692.1
Uniprot Accession Number
O95067
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
ACSM4 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
Anti-CD31/PECAM1 Antibody Picoband® (monoclonal, 2D4), Cy3 Conjugate
CHST8, ID (CHST8, Carbohydrate sulfotransferase 8, GalNAc-4-O-sulfotransferase 1, N-acetylgalactosamine-4-O-sulfotransferase 1) (Azide free) (HRP) discontinued
