Recombinant human SIL1 protein

Nucleotide exchange factor, also known as SIL1, is a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Recombinant human SIL1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nucleotide exchange factor SIL1, BAP, MSS, uLG5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

50 kDa (439aa)

Additionnal Information

SIL1, Nucleotide exchange factor SIL1, BAP, MSS, uLG5, ATGP1580-10 µg, ATGP1580-20 µg, ATGP1580-50 µg, ATGP1580-100 µg, ATGP1580-250 µg, ATGP1580-500 µg, ATGP1580-1 mg, ATGP1580-10, ATGP1580-20, ATGP1580-50, ATGP1580-100, ATGP1580-250, ATGP1580-500, ATGP1580-1

References & Citations

Chung KT., et al. (2002) J Biol Chem. 277 (49) :47557-63.; ; Senderek J., et al. (2005) Nat Genet. 37 (12) : 1312-4.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_071909

Uniprot Accession Number

Q9H173

Species

Human

AA Sequence

MHQNLKEFAL TNPEKSSTKE TERKETKAEE ELDAEVLEVF HPTHEWQALQ PGQAVPAGSH VRLNLQTGER EAKLQYEDKF RNNLKGKRLD INTNTYTSQD LKSALAKFKE GAEMESSKED KARQAEVKRL FRPIEELKKD FDELNVVIET DMQIMVRLIN KFNSSSSSLE EKIAALFDLE YYVHQMDNAQ DLLSFGGLQV VINGLNSTEP LVKEYAAFVL GAAFSSNPKV QVEAIEGGAL QKLLVILATE QPLTAKKKVL FALCSLLRHF PYAQRQFLKL GGLQVLRTLV QEKGTEVLAV RVVTLLYDLV TEKMFAEEEA ELTQEMSPEK LQQYRQVHLL PGLWEQGWCE ITAHLLALPE HDAREKVLQT LGVLLTTCRD RYRQDPQLGR TLASLQAEYQ VLASLELQDG EDEGYFQELL GSVNSLLKEL RLEHHHHHH