Recombinant human GAP-43 protein

Growth associated protein 43, also known as GAP43, is a phosphoprotein of the presynaptic membrane involved in synaptic reorganization and neural development. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. Also, GAP43 plays an essential role in the maintenance mechanism of long-term potentiation (LTP) . Recombinant human GAP43 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Growth associated protein 43, Neuromodulin, B-50, PP46

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.3 kDa (262aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

GAP43, Growth associated protein 43, Neuromodulin, B-50, PP46, ATGP1579-10 µg, ATGP1579-20 µg, ATGP1579-50 µg, ATGP1579-100 µg, ATGP1579-250 µg, ATGP1579-500 µg, ATGP1579-1 mg, ATGP1579-10, ATGP1579-20, ATGP1579-50, ATGP1579-100, ATGP1579-250, ATGP1579-500, ATGP1579-1

References & Citations

Dent E W., et al. (1998) J Neurobiol. 35:287-299.; ; Neve R L., et al. (1998) J Neurosci. 18: 7757-7767.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_002036.1

Uniprot Accession Number

P17677

Species

Human

AA Sequence

MLCCMR RTKQVEKNDD DQKIEQDGIK PEDKAHKAAT KIQASFRGHI TRKKLKGEKK DDVQAAEAEA NKKDEAPVAD GVEKKGEGTT TAEAAPATGS KPDEPGKAGE TPSEEKKGEG DAATEQAAPQ APASSEEKAG SAETESATKA STDNSPSSKA EDAPAKEEPK QADVPAAVTA AAATTPAAED AAAKATAQPP TETGESSQAE ENIEAVDETK PKESARQDEG KEEEPEADQE HA