Recombinant human RPS3A protein

40S ribosomal protein S3a, also known as RPS3A, belongs to the S3AE family of ribosomal proteins. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a ribosomal protein that is a component of the 40S subunit and is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Recombinant human RPS3A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

40S ribosomal protein S3a, FTE1, MFTL, S3A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.5 kDa (288aa) confirmed by MALDI-TOF

Additionnal Information

RPS3A, 40S ribosomal protein S3a, FTE1, MFTL, S3A, ATGP1578-10 µg, ATGP1578-20 µg, ATGP1578-50 µg, ATGP1578-100 µg, ATGP1578-250 µg, ATGP1578-500 µg, ATGP1578-1 mg, ATGP1578-10, ATGP1578-20, ATGP1578-50, ATGP1578-100, ATGP1578-250, ATGP1578-500, ATGP1578-1

References & Citations

Wool IG., et al. (1995) Biochem Cell Biol. 73 (11) :933-47.; ; Nolte D., et al. (1996) Gene. 169 (2) : 179-85.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000997

Uniprot Accession Number

P61247

Species

Human

AA Sequence

MAVGKN KRLTKGGKKG AKKKVVDPFS KKDWYDVKAP AMFNIRNIGK TLVTRTQGTK IASDGLKGRV FEVSLADLQN DEVAFRKFKL ITEDVQGKNC LTNFHGMDLT RDKMCSMVKK WQTMIEAHVD VKTTDGYLLR LFCVGFTKKR NNQIRKTSYA QHQQVRQIRK KMMEIMTREV QTNDLKEVVN KLIPDSIGKD IEKACQSIYP LHDVFVRKVK MLKKPKFELG KLMELHGEGS SSGKATGDET GAKVERADGY EPPVQESV