Recombinant human MYL12B protein

Myosin, light chain 12B, regulatory, also known as MYL12B, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL12B regulates contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK) . Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases Actin-activated myosin ATPase activity, thereby regulating contractile activity. Recombinant human MYL12B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Myosin light chain 12B regulatory, Myosin, light chain 12B regulatory, MLC-B, MRLC2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.3 kDa (196aa) confirmed by MALDI-TOF

Additionnal Information

MYL12B, Myosin light chain 12B regulatory, Myosin, light chain 12B regulatory, MLC-B, MRLC2, ATGP1577-10 µg, ATGP1577-20 µg, ATGP1577-50 µg, ATGP1577-100 µg, ATGP1577-250 µg, ATGP1577-500 µg, ATGP1577-1 mg, ATGP1577-10, ATGP1577-20, ATGP1577-50, ATGP1577-100, ATGP1577-250, ATGP1577-500, ATGP1577-1

References & Citations

Kumar C C., et al. (1989) Biochemistry. 28:4027-4035.; ; Kolodney M S., et al. (1999) J Physiol. 515: 87-92.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Scientific Category

Actin & Myosin Related

NCBI Accession Number

NP_001138417

Uniprot Accession Number

O14950

Species

Human

AA Sequence

MSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD