Recombinant human NPM1 protein

NPM1 is a phosphoprotein which moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in NPM1 are associated with acute myeloid leukemia. Recombinant human NPM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nucleophosmin, B23, NPM

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.7 kDa (314aa) confirmed by MALDI-TOF

Additionnal Information

NPM1, ATGP1576-10 µg, ATGP1576-20 µg, ATGP1576-50 µg, ATGP1576-100 µg, ATGP1576-250 µg, ATGP1576-500 µg, ATGP1576-1 mg, ATGP1576-10, ATGP1576-20, ATGP1576-50, ATGP1576-100, ATGP1576-250, ATGP1576-500, ATGP1576-1

References & Citations

Sagawa, F., et al. (2011) EMBO J. 30 (19), 3994-4005.; ; Neubauer, A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042.

Other References

Nakatomi K, et al. TLR4/MD-2 is a receptor for extracellular nucleophosmin 1. (Biomed Rep. 2021) {https://pubmed.ncbi.nlm.nih.gov/33335727/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl,1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002511.1

Uniprot Accession Number

P06748

Species

Human

AA Sequence

MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEEE EDVKLLSISG KRSAPGGGSK VPQKKVKLAA DEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL