Recombinant human NRK1/NMRK1 protein

NRK1 belongs to the uridine kinase family. NAD+ is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADPribosyl groups used in various reactions, including those that retard aging in experimental systems. Nicotinic acid and nicotinamide were defined as the vitamin precursors of NAD+. This enzyme catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN) . The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine. Recombinant human NRK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nicotinamide riboside kinase 1 isoform 1, bA235O14.2, NRK1, RP11-235O14.2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.6 kDa (222aa) confirmed by MALDI-TOF

Additionnal Information

NRK1, nicotinamide riboside kinase 1 isoform 1, bA235O14.2, NRK1, RP11-235O14.2, ATGP1570-10 µg, ATGP1570-20 µg, ATGP1570-50 µg, ATGP1570-100 µg, ATGP1570-250 µg, ATGP1570-500 µg, ATGP1570-1 mg, ATGP1570-10, ATGP1570-20, ATGP1570-50, ATGP1570-100, ATGP1570-250, ATGP1570-500, ATGP1570-1

References & Citations

Bieganowski P, et al. (2004) Cell. 14;117 (4) :495-502.; ; Khan JA, et al. (2007) Structure. 15 (8) :1005-13.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_060351.1

Uniprot Accession Number

Q9NWW6

Species

Human

AA Sequence

MKTFIIG ISGVTNSGKT TLAKNLQKHL PNCSVISQDD FFKPESEIET DKNGFLQYDV LEALNMEKMM SAISCWMESA RHSVVSTDQE SAEEIPILII EGFLLFNYKP LDTIWNRSYF LTIPYEECKR RRSTRVYQPP DSPGYFDGHV WPMYLKYRQE MQDITWEVVY LDGTKSEEDL FLQVYEDLIQ ELAKQKCLQV TA