Recombinant human NRK1/NMRK1 protein
NRK1 belongs to the uridine kinase family. NAD+ is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADPribosyl groups used in various reactions, including those that retard aging in experimental systems. Nicotinic acid and nicotinamide were defined as the vitamin precursors of NAD+. This enzyme catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN) . The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine. Recombinant human NRK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Nicotinamide riboside kinase 1 isoform 1, bA235O14.2, NRK1, RP11-235O14.2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.6 kDa (222aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bieganowski P, et al. (2004) Cell. 14;117 (4) :495-502.; ; Khan JA, et al. (2007) Structure. 15 (8) :1005-13.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_060351.1
Uniprot Accession Number
Q9NWW6
Species
Human
AA Sequence
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