Recombinant human MSRA protein

MSRA (methionine sulfoxide reductase A) belongs to the MsrA Met sulfoxide reductase family. This enzyme has an important function as a repair enzyme for proteins that have been inactivated by oxidation. It catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. In enzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3 substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, whereas its two products are peptide-L-methionine (R) -S-oxide and thioredoxin. Recombinant human MSRA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Methionine sulfoxide reductase A, bA235O14.2, NRK1, RP11-235O14.2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.2 kDa (237aa) confirmed by MALDI-TOF

Additionnal Information

MSRA, methionine sulfoxide reductase A, bA235O14.2, NRK1, RP11-235O14.2, ATGP1569-10 µg, ATGP1569-20 µg, ATGP1569-50 µg, ATGP1569-100 µg, ATGP1569-250 µg, ATGP1569-500 µg, ATGP1569-1 mg, ATGP1569-10, ATGP1569-20, ATGP1569-50, ATGP1569-100, ATGP1569-250, ATGP1569-500, ATGP1569-1

References & Citations

Boschi-Muller S, et al. (2005) Biochim. Biophys. Acta. 1703 (2) : 231-8. ; ; Hansel A., et al. (2005) Biochim. Biophys. Acta 1703:239-247.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_036463

Uniprot Accession Number

Q9UJ68

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGA ERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMR QGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNG YCGLGGTGVS CPVGIKK