Recombinant human MSRA protein
MSRA (methionine sulfoxide reductase A) belongs to the MsrA Met sulfoxide reductase family. This enzyme has an important function as a repair enzyme for proteins that have been inactivated by oxidation. It catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. In enzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3 substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, whereas its two products are peptide-L-methionine (R) -S-oxide and thioredoxin. Recombinant human MSRA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Methionine sulfoxide reductase A, bA235O14.2, NRK1, RP11-235O14.2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.2 kDa (237aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Boschi-Muller S, et al. (2005) Biochim. Biophys. Acta. 1703 (2) : 231-8. ; ; Hansel A., et al. (2005) Biochim. Biophys. Acta 1703:239-247.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
NP_036463
Uniprot Accession Number
Q9UJ68
Species
Human
AA Sequence
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