Recombinant human VTI1B protein
VTI1B, also known as v-SNARE, mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Recombinant human VTI1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Vesicle transport through interaction with t-SNAREs homolog 1B, v-SNARE, VTI1, VTI1-LIKE, vti1-rp1, VTI1L, VTI2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
26.3 kDa (231aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Fischer von Mollard G., et al. (1998) J. Biol. Chem. 273:2624-2630; ; Daub H., et al. (2008) Mol. Cell. 31:438-448
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006361
Uniprot Accession Number
Q9UEU0
Species
Human
AA Sequence
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