Recombinant human VTI1B protein

VTI1B, also known as v-SNARE, mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Recombinant human VTI1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Vesicle transport through interaction with t-SNAREs homolog 1B, v-SNARE, VTI1, VTI1-LIKE, vti1-rp1, VTI1L, VTI2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

26.3 kDa (231aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

VTI1B, Vesicle transport through interaction with t-SNAREs homolog 1B, v-SNARE, VTI1, VTI1-LIKE, vti1-rp1, VTI1L, VTI2, ATGP1568-10 µg, ATGP1568-20 µg, ATGP1568-50 µg, ATGP1568-100 µg, ATGP1568-250 µg, ATGP1568-500 µg, ATGP1568-1 mg, ATGP1568-10, ATGP1568-20, ATGP1568-50, ATGP1568-100, ATGP1568-250, ATGP1568-500, ATGP1568-1

References & Citations

Fischer von Mollard G., et al. (1998) J. Biol. Chem. 273:2624-2630; ; Daub H., et al. (2008) Mol. Cell. 31:438-448

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006361

Uniprot Accession Number

Q9UEU0

Species

Human

AA Sequence

MASSAAS SEHFEKLHEI FRGLHEDLQG VPERLLGTAG TEEKKKLIRD FDEKQQEANE TLAEMEEELR YAPLSFRNPM MSKLRNYRKD LAKLHREVRS TPLTATPGGR GDMKYGIYAV ENEHMNRLQS QRAMLLQGTE SLNRATQSIE RSHRIATETD QIGSEIIEEL GEQRDQLERT KSRLVNTSEN LSKSRKILRS MSRKVTTNKL L