Recombinant E.coli L-lactate dehydrogenase/LLDD protein

LldD, also known as L-lactate dehydrogenase, is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD) . Recombinant E. coli lldD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

L-lactate dehydrogenase, ECK3595, JW3580, lct, lctD

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.3 kDa (420aa) confirmed by MALDI-TOF

Additionnal Information

LldD, L-lactate dehydrogenase, ECK3595, JW3580, lct, lctD, ATGP1563-10 µg, ATGP1563-20 µg, ATGP1563-50 µg, ATGP1563-100 µg, ATGP1563-250 µg, ATGP1563-500 µg, ATGP1563-1 mg, ATGP1563-10, ATGP1563-20, ATGP1563-50, ATGP1563-100, ATGP1563-250, ATGP1563-500, ATGP1563-1

References & Citations

Ogura Y., et al. (2009) Proc. Natl. Acad. Sci. u.S.A. 106:17939-17944

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418062

Uniprot Accession Number

P33232

Species

E.coli

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMIISAA SDYRAAAQRI LPPFLFHYMD GGAYSEYTLR RNVEDLSEVA LRQRILKNMS DLSLETTLFN EKLSMPVALA PVGLCGMYAR RGEVQAAKAA DAHGIPFTLS TVSVCPIEEV APAIKRPMWF QLYVLRDRGF MRNALERAKA AGCSTLVFTV DMPTPGARYR DAHSGMSGPN AAMRRYLQAV THPQWAWDVG LNGRPHDLGN ISAYLGKPTG LEDYIGWLGN NFDPSISWKD LEWIRDFWDG PMVIKGILDP EDARDAVRFG ADGIVVSNHG GRQLDGVLSS ARALPAIADA VKGDIAILAD SGIRNGLDVV RMIALGADTV LLGRAFLYAL ATAGQAGVAN LLNLIEKEMK VAMTLTGAKS ISEITQDSLV QGLGKELPAA LAPMAKGNAA