Recombinant human eIF-4H/EIF4H protein

Eukaryotic translation initiation factor 4H, also known as EIF4H, is a 248 amino acid protein that localizes to the perinuclear region of the cytoplasm and is expressed as two isoforms, designated short and long. EIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors EIF4A and EIF4B. EIF4H was further shown to stimulate the initial rate and extent of EIF4A-mediated mRNA secondary structure unwinding. Defects in the gene encoding EIF4H are associated with Williams- Beuren syndrome (WBS), a rare developmental disorder characterized by cardiovascular and musculo-skeletal abnormalities. Recombinant human EIF4H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 4H, eIF-4H, WBSCR1, WSCR1, Williams-Beuren syndrome chromosome region 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.9 kDa (272aa) confirmed by MALDI-TOF

Additionnal Information

EIF4H, Eukaryotic translation initiation factor 4H, eIF-4H, WBSCR1, WSCR1, ATGP1562-10 µg, ATGP1562-20 µg, ATGP1562-50 µg, ATGP1562-100 µg, ATGP1562-250 µg, ATGP1562-500 µg, ATGP1562-1 mg, ATGP1562-10, ATGP1562-20, ATGP1562-50, ATGP1562-100, ATGP1562-250, ATGP1562-500, ATGP1562-1

References & Citations

Richter Cook N J., et al. (1998) J Biol Chem. 273:7579-7587.; ; Doepker R C., et al. (2004) J Virol. 78: 4684-4699.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_071496

Uniprot Accession Number

Q15056

Species

Human

AA Sequence

MADFDT YDDRAYSSFG GGRGSRGSAG GHGSRSQKEL PTEPPYTAYV GNLPFNTVQG DIDAIFKDLS IRSVRLVRDK DTDKFKGFCY VEFDEVDSLK EALTYDGALL GDRSLRVDIA EGRKQDKGGF GFRKGGPDDR GMGSSRESRG GWDSRDDFNS GFRDDFLGGR GGSRPGDRRT GPPMGSRFRD GPPLRGSNMD FREPTEEERA QRPRLQLKPR TVATPLNQVA NPNSAIFGGA RPREEVVQKE QE