Recombinant human CLTA protein

Clathrin, light chain A, also known as CLTA, belongs to the clathrin family. Clathrin is a large, soluble protein composed of heavy and light chains. It is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are captured by Clathrin-coated vesicles. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements. Recombinant human CLTA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Clathrin light chain A, Clathrin, light chain A, LCA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.2 kDa (242aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CLTA, Clathrin light chain A, Clathrin, light chain A, LCA, ATGP1560-10 µg, ATGP1560-20 µg, ATGP1560-50 µg, ATGP1560-100 µg, ATGP1560-250 µg, ATGP1560-500 µg, ATGP1560-1 mg, ATGP1560-10, ATGP1560-20, ATGP1560-50, ATGP1560-100, ATGP1560-250, ATGP1560-500, ATGP1560-1

References & Citations

Kirchhausen T., et al. (1987) Proc Natl Acad Sci uSA. 84:8805-8809.; ; Liu S H., et al. (1995) Cell. 83: 257-267.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001824

Uniprot Accession Number

P09496

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAELDP FGAPAGAPGG PALGNGVAGA GEEDPAAAFL AQQESEIAGI ENDEAFAILD GGAPGPQPHG EPPGGPDAVD GVMNGEYYQE SNGPTDSYAA ISQVDRLQSE PESIRKWREE QMERLEALDA NSRKQEAEWK EKAIKELEEW YARQDEQLQK TKANNRAAEE AFVNDIDESS PGTEWERVAR LCDFNPKSSK QAKDVSRMRS VLISLKQAPL VH