Recombinant human HAAO protein
HAAO is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. This protein catalyzes the synthesis of quinolinic acid (QuIN) from 3-hydroxyanthranilic acid. QuIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QuIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QuIN. Recombinant human HAAO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
3-hydroxyanthranilate 34-dioxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, 3-HAO, HAO
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by BCA assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35 kDa (310aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Malherbe P., et al. (1994) J Biol Chem. 13; ;269 (19) :13792-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_036337
Uniprot Accession Number
P46952
Species
Human
AA Sequence
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