Recombinant human JAM-A/F11R protein

F11R, also known as CD321, belongs to the immunoglobulin superfamily. It seems to plays a role in epithelial tight junction formation. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. F11R protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Recombinant human F11R protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, JAM-1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, F11R, JAM1, JCAM

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.8 kDa (238aa) confirmed by MALDI-TOF

Additionnal Information

JAM-A, Junctional adhesion molecule A, CD321, JAM, JAM1, JAMA, JCAM, KAT, PAM-1, ATGP1556-10 µg, ATGP1556-20 µg, ATGP1556-50 µg, ATGP1556-100 µg, ATGP1556-250 µg, ATGP1556-500 µg, ATGP1556-1 mg, ATGP1556-10, ATGP1556-20, ATGP1556-50, ATGP1556-100, ATGP1556-250, ATGP1556-500, ATGP1556-1

References & Citations

Murakami, M., et al. (2011) PLoS ONE 6 (6), E21242

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.15M NaCl,1mM DTT

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_058642

Uniprot Accession Number

Q9Y624

Species

Human

AA Sequence

LGSVT VHSSEPEVRI PENNPVKLSC AYSGFSSPRV EWKFDQGDTT RLVCYNNKIT ASYEDRVTFL PTGITFKSVT REDTGTYTCM VSEEGGNSYG EVKVKLIVLV PPSKPTVNIP SSATIGNRAV LTCSEQDGSP PSEYTWFKDG IVMPTNPKST RAFSNSSYVL NPTTGELVFD PLSASDTGEY SCEARNGYGT PMTSNAVRME AVERNVGV