Recombinant human AS3MT protein

AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. Recombinant human AS3MT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Arsenic (+3 oxidation state) methyltransferase, CYT19, RP11-753C18.6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.3 kDa (399aa) confirmed by MALDI-TOF

Additionnal Information

AS3MT, Arsenic (+3 oxidation state) methyltransferase, CYT19, RP11-753C18.6, ATGP1555-10 µg, ATGP1555-20 µg, ATGP1555-50 µg, ATGP1555-100 µg, ATGP1555-250 µg, ATGP1555-500 µg, ATGP1555-1 mg, ATGP1555-10, ATGP1555-20, ATGP1555-50, ATGP1555-100, ATGP1555-250, ATGP1555-500, ATGP1555-1

References & Citations

Sumi, D., et al. (2011) Biochem. Biophys. Res. Commun. 415 (1), 48-53 ; ; Watanabe, T., et al. (2011) Arch. Toxicol. 85 (6), 577-588

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.15M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_065733

Uniprot Accession Number

Q9HBK9

Species

Human

AA Sequence

MAALRD AEIQKDVQTY YGQVLKRSAD LQTNGCVTTA RPVPKHIREA LQNVHEEVAL RYYGCGLVIP EHLENCWILD LGSGSGRDCY VLSQLVGEKG HVTGIDMTKG QVEVAEKYLD YHMEKYGFQA SNVTFIHGYI EKLGEAGIKN ESHDIVVSNC VINLVPDKQQ VLQEAYRVLK HGGELYFSDV YTSLELPEEI RTHKVLWGEC LGGALYWKEL AVLAQKIGFC PPRLVTANLI TIQNKELERV IGDCRFVSAT FRLFKHSKTG PTKRCQVIYN GGITGHEKEL MFDANFTFKE GEIVEVDEET AAILKNSRFA QDFLIRPIGE KLPTSGGCSA LELKDIITDP FKLAEESDSM KSRCVPDAAG GCCGTKKSC